background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

abstract background: fertilization failure is one of the most problems in assisted reproduction technology (art). objective: the aim of this study was the evaluation of oocytes activation by addition of calcium ionophore in unfertilized oocytes in icsi cycles. materials and methods: this study was done on 15 icsi cycles (stimulated with standard long protocol). mature retrieved oocytes with nor...

Background: The advances in cytogenetic techniques during the last few years have permitted not only the study of large populations of wild and domestic animals, but also the detection of chromosome anomalies in embryos. Chromosomal abnormalities are the most common cause of embryonic and fetal mortality in mammals. Most reports of chromosome anomalies in parthenogenetic embryos describe numeri...

Background The role of calcium in physiological phenomenona such as sperm acrosome reaction and hyper-activation has been well known. Calcium ionophore A23187 is routinely used for induction of acrosome reaction in mammalians sperm. Therefore, we decided to evaluate the effect of calcium ionophore A23187 on the sperm hyper-active motility patterns. MaterialsAndMethods Ten semen samples from ind...

Novel and effective calcium ion binding molecular fluorescent probes have been developed by systematic structural modification of BAPTA ionophore. The comprehensive computational studies suggested the role coordination number in chelator moiety.

Background: Parthenogenetic activation of mammalian oocytes using artificial stimuli is commonly used in various reproductive bio-techniques. Calcium ionophore is known to elevate intracellular calcium levels in the cytoplasm of oocytes through the influx of calcium from extracellular spaces. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial ...

background: globozoospermia is a rare (incidence of 0.1% among andrological patients) and poorly understood condition, but a severe disorder in male infertility. this case report detailed the course of treatment and protocol of a patient with type 1 globozoospermia using intracytoplasmic sperm injection (icsi) and oocyte activation by calcium ionophore, which yielded conception and birth of a h...

Background: Parthenogenetic activation is a possible way to produce homogeneous embryos with the some ploidy. Probably such embryos could be used in other areas of biotechnology. Calcium signals are known as important regulators of oocyte activation. Extracellular calcium is required for initiation of meiotic resumption and development. Calcium ionophore A23187 is known to elevate intracellular...

oocyte activation (aoa), results in high fertilization rates. objective: this prospective, randomized, unblinded clinical study aimed to evaluate the efficiency of calcium ionophore on fertilization and pregnancy rate after intracytoplasmic sperm injection (icsi) in infertile men suffer from teratoospermia. materials and methods: thirty eight women with teratoospermic partner underwent icsi wit...

background: activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. this study was conducted to determine microparticles formation and platelet shrinkage ...